Fig. 2

ETV2 upregulates miR126 expression through direct binding on Egfl7 promoter. a Expression analysis. iFLAG-ETV2 mESCs were differentiated for 3.5 days ± Dox treatment and were subjected to gene expression analysis. n = 3, *p < 0.05. b Schematic diagram of Egfl7 promoter-luciferase plasmid. Two potential ETS binding elements were marked as diamonds, and their mutant sequences were shown in the bottom. c HEK/293T cells were transiently co-transfected with pCMV-Etv2 and pGL3-luciferase constructs carrying wild type (Wt), or mutants of Egfl7 promoter. Firefly luciferase activity was normalized by Renilla luciferase activity. n = 3. ***p < 0.001. d iFLAG-ETV2 mESCs were differentiated in the presence of Dox for 3.5 days and then subjected to ChIP-PCR assay. Rabbit anti-FLAG or IgG antibody was used for the immunoprecipitation. n = 3, **p < 0.01