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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Autofluorescence of NADH is a new biomarker for sorting and characterizing cancer stem cells in human glioma

Fig. 4

Both NADHhigh and CD133+ glioma cells possess the properties of GSCs, but are independent each other. a qRT-PCR analysis showing upregulated stemness-related transcription factor genes Nanog, Oct4, Oligo2, and Sox2 in NADHhigh CD133+, NADHhigh CD133, and NADHlow CD133+ subpopulations, compared to NADHlow CD133 in GBM1 and LN229. b, c Limiting dilution assay showed increased sphere formation rate and sphere average diameter of NADHhigh/CD133+, NADHhigh/CD133, and NADHlow/CD133+ subpopulations, compared to NADHlow CD133cells in GBM1 and LN229 cell lines. All data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 independent experiments)

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