Fig. 5

Immunofluorescence analysis of hASCs transfected with let-7i-3p mimics and inhibitor. a Compared to that in normal cultured hASCs, the immunofluorescence intensity of LEF1 was decreased in hASCs transfected with let-7i-3p mimics. Compared with that in normal cultured hASCs, the immunofluorescence intensity of LEF1 was increased in hASCs transfected with the let-7i-3p inhibitor. b Compared to that in normal cultured hASCs, the immunofluorescence intensity of β-catenin was decreased in both the cytoplasm and nucleus of hASCs transfected with let-7i-3p mimics. Compared to that in normal cultured hASCs, the immunofluorescence intensity of β-catenin in both the cytoplasm and nucleus was promoted after transfection of the let-7i-3p inhibitor (scale bar 100 μm)