Fig. 7

Effects of β-catenin modulation on the osteogenic differentiation of hASCs under cyclic strain (*p < 0.05, significant differences existed between these two groups). a Transfection efficiency detected by qPCR. The expression of β-catenin in the EX-β-catenin group was increased 3.71 ± 0.42-fold (p < 0.001) compared to that in the EX-Ctrl group. The expression of β-catenin in the siβ-catenin group was significantly decreased 3.00 ± 0.76-fold (p = 0.002) compared to that in the siR-Ctrl group. b The effect of β-catenin overexpression on the osteogenic differentiation of hASCs under cyclic strain as determined by qPCR. Compared to those in the EX-Ctrl group, the levels of the following mRNA markers in hASCs transfected with EX-β-catenin under cyclic strain for 6 days were significantly increased: RUNX2 (4.73 ± 1.60-fold, p = 0.001), ALP (3.76 ± 2.04-fold, p = 0.021), SPARC (1.85 ± 0.49-fold, p = 0.004), LEF1 (2.64 ± 0.40-fold, p = 0.006), and β-catenin (4.30 ± 1.12-fold, p = 0.001). c The effect of β-catenin suppression on the osteogenic differentiation of hASCs under cyclic strain as determined by qPCR. Compared to those in the miR-Ctrl inhibitor group, the levels of the following mRNA markers in hASCs transfected with EX-β-catenin under cyclic strain for 6 days were significantly decreased: RUNX (2.61 ± 0.45-fold, p < 0.001), ALP (3.46 ± 0.79-fold, p = 0.009), SPARC (2.16 ± 0.02-fold, p = 0.010), LEF1 (2.52 ± 0.61-fold, p = 0.001), and β-catenin (4.15 ± 1.72-fold, p = 0.004)