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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Alteration of fatty acid oxidation by increased CPT1A on replicative senescence of placenta-derived mesenchymal stem cells

Fig. 5

Effect of increased CPT1A in senescent PD-MSCs during long-term cultivation. a The levels of CPT1A and ACC mRNA were determined by using qRT-PCR. b Protein levels of p-AMPK/AMPK, p-ACC/ACC, PPARα, and CPT1A related to fatty acid pathway of early and late passage PD-MSCs were analyzed by Western blotting. c SA-β-galactosidase activity of late passage PD-MSCs treated with CPT1A siRNA was assayed by SA-β-galactosidase staining (scale bar, × 40). The SA-β-galactosidase stained cells were counted by Image J software. The data were representative of three independent experiments and expressed as means ± S.D. An asterisk indicates P < 0.05 versus early passage and control group

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