Fig. 4

Multiple ADSC infusions attenuated liver disease in long-term T2DM complication rats. a Representative liver sections staining with H&E, Sirius Red, and Oil Red O; bars = 50, 50, 50, and 20 μm. b Levels of serum ALT, AST, and ALP of each group were evaluated. c Levels of serum LDL-C, TC, and TG of each group were evaluated. d Histopathological damage scores were graded using H&E-stained sections. e Ratio of Sirius Red-positive area to total area. f, g Immunofluorescence and quantification of α-SMA and collagen I area; bars = 100 μm. h, i Quantitative reverse transcriptase polymerase chain reaction analysis of gene expression in liver tissue from control, T2DM, and MSC groups; results are presented relative to those of control rats, set as 1. N = 6 rats per group; *p < 0.05, **p < 0.01