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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway

Fig. 2

AML cell-secreted exosomes target HSCs to mediate hematopoiesis. a, b Uptake of CFSE-labeled exosomes by CD34+ HSCs, as detected under the fluorescence microscope. (×400) c Colony-forming capacity of CD34+ HSCs 48 h after culture with different dose of Molm-14 cell-derived exosomes. d Relative expression of hematopoietic suppressor gene DKK1 in CD34+ HSCs after culture with different dose of Molm-14 cell-derived exosomes measured by RT-qPCR. *p < 0.05 vs. the treatment of PBS. Measurement data were described as mean ± standard deviation. Comparisons between two groups were analyzed by unpaired t test. The cell experiment was repeated three times to obtain the mean value. miR-4532, microRNA-4532; LDOC1, leucine-zipper downregulated in cancer 1; DKK1, dickkopf-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-qPCR, reverse transcription quantitative polymerase chain reaction

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