Fig. 4

AML cell-derived exosomes overexpressing miR-4532 suppress normal hematopoiesis by targeting LDOC1. a The colony-forming capacity of CD34⁺ HSCs 48 h after culture with transfected Molm-14 cell-derived exosomes. b The relative expression of hematopoietic suppressor gene DKK1 in CD34⁺ HSCs after culture with transfected Molm-14 cell-derived exosomes measured by RT-qPCR. *p < 0.05 vs. CD34⁺ HSCs co-cultured with exosomes from inhibitor-NC-treated Molm-14 cells. c Colony-forming capacity of CD34⁺ HSCs after alteration of miR-4532 and LDOC1. d The relative expression of hematopoietic suppressor gene DKK1 in CD34⁺ HSCs after alteration of miR-4532 and LDOC1 measured by RT-qPCR. *p < 0.05 vs. CD34⁺ HSCs treated with mimic-NC, ^#p < 0.05 vs. CD34⁺ HSCs cultured with miR-4532 mimic, ^&p < 0.05 vs. CD34⁺ HSCs cultured with si-NC. Measurement data were described as mean ± standard deviation. Comparisons between two groups were analyzed by unpaired t test, and comparisons among multiple groups were analyzed by one-way analysis of variance (ANOVA), followed by Tukey’s post hoc test. The cell experiment was repeated three times to obtain the mean value. miR-4532, microRNA-4532; LDOC1, leucine-zipper downregulated in cancer 1; RT-qPCR, reverse transcription quantitative polymerase chain reaction