Fig. 4

Gene and protein expression of myosin in injury site of the external anal sphincter after laser, human adipose-derived stem cells (hADSCs), and combination of laser and hADSCs (laser + hADSCs) treatments. hADSCs labeled by DiI (red). Myosin expression is observed in the hADSCs and laser groups (green). Data are presented as means ± SEM (n = 4 animal per group). ***Significant level at p < 0.0001 with the sphincterotomy group. **Significant level at p < 0.01 with the sphincterotomy group. ^###Significant level at p < 0.0001 with the control group (intact animals). ^$$$Significant level at p < 0.0001 with the laser + hADSCs group. ^$$Significant level at p < 0.01 with the laser + hADSCs group. ^†Significant level at p < 0.05 with the hADSCs group. Real-time PCR, immunohistochemistry, and western blotting, external anal sphincter, rabbit (× 10)