Fig. 5

Metformin treatment increases the number of Pax7+ cells and protein level at 7 days in the skeletal muscle after severe burn injury. a Representative western blot for Pax7 and GAPDH protein. b Quantification of Pax7 protein level normalized to GAPDH. c Representative images of Pax7 immunohistochemistry in sham, burn, and burn + metformin mice at 7 days post-burn injury. Arrows indicate positive cells, and arrowheads show negative cells. Images were obtained at × 40 magnification. d Quantification of Pax7-positive nuclei in sham, burn, and burn + metformin mice at 7 days post-burn injury