Fig. 6

Transplantation of SSCs. Trypan blue staining was used to show the SSC transplantation procedure. A 3-mm incision was made at the dorsal tip of the testis tissue of the recipient mouse, and the seminiferous tubules were exposed. After the injection, the trypan blue solution gradually filled along the seminiferous tubules. One injection (40–50 μL) could fill one fourth to one third of the testicular tissue. Scale bar = 1 mm