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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation

Fig. 9

Morphological changes of testicular tissue before and after transplantation. a Testicle of SSC transplantation recipient; it can be seen that spermatogenic cells in testicular tissue disappeared. b After 12 weeks of natural recovery, the spermatogenic function was still not recovered. c A large number of spermatogenic cells and sperm were found in recipient testicular tissue after 12 weeks of SSC transplantation. d–g Immunohistochemical staining for detection of GFP expression in testicular tissues. d Negative control. e Natural recovery of testicular tissue in mice after 12 weeks of treatment with busulfan. f, g Testicular tissue after 12 weeks of GFP+ SSC transplantation, a large number of GFP+ spermatogenic cells and spermatozoa in the convoluted tubule. Immunofluorescence assay staining for detection of GFP expression in testicular tissues after 12 weeks of SSC transplantation (h–k). h GFP. i DAPI. j, k Meger. Magnification, scale bar = 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512