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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Extracellular matrix derived from human urine-derived stem cells enhances the expansion, adhesion, spreading, and differentiation of human periodontal ligament stem cells

Fig. 6

Osteogenic differentiation on different substrates. a ALP, b RUNX2, c OCN, and d POSTN expression were analyzed on culture day 7 in osteogenic medium by real-time PCR. e RUNX2 and POSTN expression were analyzed on culture day 7 in osteogenic medium by western blot. f The relative density of western blot using NIH Image J. g After osteogenic induction on different substrates for 21 days, mineral deposits were detected by alizarin red staining. Scale bar = 200 μm. h Quantitation of alizarin red staining of day 21 cultures. All values represent the mean ± SD; *p < 0.05 and **p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512