Fig. 1

Induction and identification of DCs. a The morphology of DCs. Cell morphology on days 1, 3 (left and middle, monocytes in the presence of GM-CSF and IL-4), and 7 (right, imDC cultured for 24 h under LPS stimulation) (×200 magnification). b Immunophenotype analysis of DCs (expression of MHCII, CD86, and CD40 in DCs cultured for 24 h in the presence of LPS at concentrations ranging from 0 to 1000 ng/ml). c The percentage of DCs positive for MHCII, CD86, and CD40 after incubation for 24 h with LPS at concentrations ranging from 0 to 1000 ng/ml. d Immunophenotype analysis of DCs (expression of MHCII, CD86, and CD40 on DCs after culture for 0 h, 24 h, and 48 h with an LPS concentration of 50 ng/ml). e The percentage of DCs expressing MHCII, CD86, and CD40 after 0 h, 24 h, and 48 h cultured at an LPS concentration of 50 ng/ml. n = 3. c *P < 0.05 versus LPS 0 ng/ml group; #P < 0.05 versus LPS 10 ng/ml group; &P < 0.05 versus LPS 50 ng/ml group. e *P < 0.05 versus Con group; #P < 0.05 versus 24 h group. Data are expressed as mean ± SD. Each experiment was repeated three times