Fig. 2

Effects of MSCs and rhHGF on mDC differentiation. a Immunophenotype analysis of DCs (expression of MHCII, CD86, and CD40 in mDCs cultured for 72 h in the presence of MSC or rhHGF, and expression of MHCII, CD86, and CD40 in MSC-induced DCs after LPS stimulation for 24 h). Percentage of MHCII, CD86, and CD40 expression on DCs after 72 h of incubation with or without MSC or rhHGF. b Phagocytic ability analysis of DCs (expression of OVA-FITC in DCs and percentage of OVA-FITC-positive cells of DCs). c Lymphocyte proliferation stimulated by mitotic proenzyme ConA, mDCs, MSC-DCs, or rhHGF-DCs. Splenocytes from normal BALB/c mice were used as responder cells in the mitogen proliferative assay. mDCs, MSC-DCs, rhHGF-DCs, or ConA were used as stimulators. Splenocytes from normal mice served as controls. The proliferative responses were assessed by CFSE labeling and FACS (gray line, unstimulated spleen cell). d Immunophenotype analysis of DCs (expression of PD-L1 and IL-27 in mDCs cultured for 72 h in the presence of MSC or rhHGF). Percentage of PD-L1 and IL-27 expression in DCs after 72 h of incubation with or without MSC or rhHGF. e Cytokine secretion profiles of mDCs, MSC-DCs, and rhHGF-DCs after culture for 24 h. f Changes in c-met and phosphorylated c-met levels in dendritic cells cultured for 72 h in the presence of MSCs or rhHGF (n = 3, *P < 0.05 versus mDC group; data are expressed as mean ± SD). Each experiment was repeated times