Fig. 3

Long-term transgene expression efficiency in MSCs after lentiviral vector transduction. a The MSCs transduced separately with PDS087-EGFP (NC-MSCs), CL1017-PDS087_pL6-CMV-EGFP-IRES-HGF (HGF-MSCs), PDS019-EGFP (NC-shHGF-MSCs), and VL1012_pL-m-HGF-shRNA-1173 (shHGF-MSCs) lentiviral vectors cultured for 20 passages and observed with light microscopy (top) and fluorescence microscopy with green fluorescent protein (bottom), ×200. b The percentage of GFP-positive cells analyzed by flow cytometry at passage 20 after transduction (Based on untransfected MSCs in the green line, gates on the panel in the red line represent transfection-positive cells). c Quantitative real-time PCR analysis of HGF mRNA expression in MSCs after transduction. d The expression of HGF protein in MSCs after transduction evaluated using Western blot analysis. e The cytokine HGF secreted by MSCs after transduction cultured for 48 h detected by ELISA (n = 3, *P < 0.05 versus NC-HGF-MSC; #P < 0.05 versus NC-shHGF-MSC; data are expressed as mean ± SD). Each experiment was repeated three times