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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

Fig. 3

Characterization of clinical bone marrow MSCs expanded in MSCGM or NRLM. a Representative bright-field micrographs of clinical MSCs cultivated in MSCGM or NRLM. b Flow cytometry analysis of cell surface marker expression, including CD29, CD34, CD44, CD45, CD90, CD73, CD105, and CD166, in MSCGM- or NRLM-cultured MSCs. c Representative micrographs showing adipocyte differentiation potential (oil red-positive staining) from two kinds of cultivated MSCs. d Analysis of the CD90+ population in clinical MSCs cultivated in MSCGM or NRLM by flow cytometry (N = 31, including commercial MSC samples shown as yellow spots). Data are presented as the mean ± SEM. ***p < 0.001 by two-tailed t test. e MSC differentiation into adipocytes, which contain fluorescently labeled (red) oil. Bar chart showing the quantification of relative fluorescence units from 28 clinical MSC and commercial MSC samples (shown as yellow spots). Data are presented as the mean ± SEM. **p < 0.01 by two-tailed t test

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Stem Cell Research & Therapy

ISSN: 1757-6512