Fig. 5

Analysis of the neuroregenerative and neuroprotective effects of clinical MSCs cultured in MSCGM or NLRM. a Neuroregeneration assay in spinal cord neuron (seeded in transwell inserts) and MSCs (seeded in culture wells) cocultures incubated for 5 days. The density of GAP-43-positive immunoreactivity in the bottom side of the transwell following scrape injury was analyzed and is shown in the bar chart. Representative GAP-43-positive micrographs are shown (bar = 200 μm). b Neuroprotection test in spinal cord neuron cultures and MSC cocultures after H₂O₂ (120 μM for one day) treatment. The apoptotic cells in neuron cultures after coculture with MSCs and H₂O₂ treatment were processed for TUNEL staining, and representative micrographs are shown (bar = 200 μm). The bar chart shows the quantification of the TUNEL-positive cells. Data are presented as the mean ± SEM. **p < 0.01 compared with the control group using one-way ANOVA with Tukey’s multiple comparisons test