Fig. 6

Characterization of the CM derived from 2 kinds of cultivated MSCs, grouped by donor age and by cytokine array analysis. a Hierarchical clustering of the rows and columns was carried out according to the cytokine array intensity values (calculated by ImageJ software) for MSCGM-CM (M) and NRLM-CM (N) samples from the different patients (young, middle-aged, and older) using MORPHEUS one minus Pearson correlation (Versatile matrix visualization and analysis software; https://software.broadinstitute.org/morpheus). b The statistical graph shows that the levels of 6 out of 120 proteins/cytokines were significantly different between the 2 kinds of cultivated MSC-CM. A human cytokine antibody array kit from RayBiotech was used to analyze the MSC-CM, revealing the presence of 120 cytokines and trophic factors. Data are presented as the mean ± SEM. *p < 0.05 and **p < 0.01 by two-tailed t test (N = 3)