Fig. 6

Sox19b regulated NSCs through an epigenetic mechanism. a Western blot analysis of acetyl-H3 protein levels in control and Sox19b MO-injected embryos. Sox19b had no effect on the acetyl-H3 levels. b Western blot analysis of H3K27me3 and H3K9me3 levels in control and Sox19b MO-injected embryos. The H3K27me3 level decreased after knocking down sox19b, but the level of H3K9me3 remained unchanged. Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control. c, d Control and Sox19b MO-injected embryos were immunoprecipitated with anti-H3K27me3 antibody, and the isolated DNA was analysed by using gene-specific ChIP primers. Rabbit IgG was used as a negative control. DNA from each ChIP sample was normalized by the corresponding input sample. Compared with the controls, the level of H3K27me3 in the Ngn1 and ascl1a promoter regions decreased significantly in Sox19b MO-injected embryos. Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control