Fig. 4

Endometrial stromal cells license to an anti-inflammatory phenotype without upregulation of human leukocyte antigen II. Representative histograms (colored white) of eSC cell surface expression of CD119 at a baseline, b 3 days, and c 7 days of interferon (IFN)γ and tumor necrosis factor (TNF)α exposure. Gray histograms indicate respective immunoglobulin controls. Representative scatterplots of eSC cell surface expression of CD120a and CD120b at d baseline, e 3 days, and f 7 days of IFNγ and TNFα exposure. Box and whisker plots showing the minimum and maximum distribution of the median fluorescence intensity (MFI) of g CD119—phycoerythrin (PE; n = 5), h CD120a—allophycocyanin (APC; n = 5), and i CD120b-PE (n = 5). Representative histograms (colored white) of eSC cell surface expression of human leukocyte antigen (HLA) I at j baseline, k 3 days, and l 7 days of IFNγ and TNFα exposure. Gray histograms indicate respective immunoglobulin controls. m Box and whisker plots showing the minimum and maximum distribution of the MFI of HLA I—Alexa Fluor (AF) 488 (n = 5). Representative histograms (colored white) of eSC cell surface expression of HLA II at n baseline, o 3 days, and p 7 days of IFNγ and TNFα exposure (n = 5). Gray histograms indicate respective immunoglobulin controls. Box and whisker plots showing the minimum and maximum distribution of baseline and post-licensing concentrations of q L-kynurenine (as a marker of indoleamine-2,3 dioxygenase activity), r interleukin (IL)-6, and s prostaglandin (PG) E2 in conditioned cell culture media (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001