Table 1 Antibodies and Ig controls used for cell surface characterization
From: Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile
Antibodies/isotype controls | Clone | Fluorochrome | Company | Catalog number |
|---|---|---|---|---|
CD90 | 5E10 | PERCP Cy5.5 | Biolegend | 328117 |
CD73 | AD2 | APC | Abcam | ab155378 |
CD105 | MEM-229 | PE | Abcam | ab53321 |
CD45 | HI30 | PB | Biolegend | 304021 |
CD14 | HCD14 | PB | Biolegend | 325615 |
CD19 | HIB19 | PB | Biolegend | 302223 |
CD34 | 581 | PB | Biolegend | 343511 |
CD120a | W15099A | APC | Biolegend | 369906 |
CD120b | REA520 | PE | Miltenyi Biotec | 130107705 |
CD119 | GIR-94 | PE | Biolegend | 308703 |
HLA II | CR3/43 | FITC | Dako | F0817 |
HLA I | W6/32 | AF 488 | Biolegend | 311413 |
CD3 | UCHT1 | V450 | BD Bioscience | 560365 |
CD4 | RPA-T4 | PERCP Cy5.5 | BD Bioscience | 560650 |
CD25 | M-A251 | PE | BD Bioscience | 555432 |
CD27 | M-T271 | AF700 | BD Bioscience | 560611 |
CD127 | HIL-7R-M21 | AF647 | BD Bioscience | 558598 |
CD45RA | HI100 | PE | Biolegend | 304107 |
Mouse IgG1 /IgG2a | Â | PE/FITC | BD Bioscience | 342409 |
Mouse IgG1, κ | 15H6 | APC | Abcam | ab37391 |
Mouse IgG1, κ | MOPC-21 | PB | Biolegend | 400131 |
Mouse IgG1, κ | MOPC-21 | PerCP Cy5.5 | Biolegend | 400149 |
Mouse IgG1, κ | 11711 | PE | R&D Systems | IC002P |
Mouse IgG2a | X5563 | PE | Abcam | ab91363 |