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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction

Fig. 2

P2X7 expression in h-MSCs under regular culture and osteogenic differentiation. a qRT-PCR analysis of P2X7 expression in h-MSCs cultured in basic medium, basic medium+15 Hz/1 mT EMF, osteogenic medium, and osteogenic medium+15 Hz/1 mT EMF for 0, 1, 3, 7, 14, and 21 days. P2X7 mRNA expression was normalized against GAPDH. Western blot analysis of P2X7 expression in h-MSCs treated with b basic medium, basic medium+15 Hz/1 mT EMF, c osteogenic medium and osteogenic medium+15 Hz/1 mT EMF for 0, 1, 3, 7, 14, and 21 days. P2X7 protein expression was normalized against GAPDH. Data represent means ± SD. Statistically significant differences are indicated; n = 3; *P < 0.05, vs. control

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Stem Cell Research & Therapy

ISSN: 1757-6512