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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction

Fig. 4

Activation of the Akt/GSK-3β/β-catenin signaling pathway in osteogenic differentiated h-MSCs by upregulated P2X7 expression induced by EMF exposure. a Western blot analysis of the effect of EMF on protein expression of p-Akt (Ser473), total Akt, p-GSK3β (Ser9), total GSK3β, and nuclear β-catenin in the presence of A740003 or/and LY294002. b qRT-PCR analysis of the effect of EMF exposure in the presence of A740003 or/and LY294002 on mRNA expression of RUNX2, ALP, and OPN. c Western blot analysis of the effect of EMF in the presence of A740003 or/and LY294002 on protein expression of RUNX2 and OPN. d Alizarin red S staining of osteogenic induced h-MSCs exposed to EMF in the presence of A740003 or/and LY294002 for 7 days. Data represent means ± SD. Statistically significant differences are indicated; n = 3; *P < 0.05, vs. control

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