Fig. 1

Influence of C. glomerata extract on EqASC condition. a Histograms represent the average absorbance at 600 nm of the metabolized rezasurin dye by treated and untreated living cells. b Bar charts represent the mean of the absorbance of the BrdU level incorporated in the neo-synthesized DNA of the treated and untreated cells. c Clonogenic fibroblast precursor (CFU-F) assay for treated and untreated ESCs. d Representative photomicrographs for Ki-67 immunostaining after extract treatment. e DAPI, MitoRed, and Phalloidin labeled cells were observed using an inverted epi-fluorescent confocal microscope; scale bar size 20 μm; magnification was set at 60-fold. Cell surface and shape was assessed by the mean of scanning electron microscope; scale bar size 30 μm; images were acquired under 1000-fold magnification. f Bar charts representation of corrected total cell fluorescence (CTCF) for Ki-67, Phalloidin and MitoRed fluorescent staining. The results are expressed as the mean of 3 different experiments ± SD. Asterisk (*) refers to comparison of treated groups to untreated EMS cells. Hashtag (#) refers to comparison of treated groups to untreated healthy cells. *^/#p < 0.05, **^/##p < 0.01, ***/^###p < 0.001