Fig. 3
From: Xenofree generation of limbal stem cells for ocular surface advanced cell therapy
Cell growth, viability, and clonogenicity of the LSC cultured on murine 3T3 or human PLA feeder layers. a Cell growth evaluated by WST-1 was higher in LSC grown on PLA feeder layers during culture. b Evaluation of the LSC viability by trypan blue exclusion assay did not show differences after cell detachment. c DPT was higher in LSC cultured on PLA feeder cells. d Clonogenicity was increased in LSC cultured on PLA feeder cells (n = 6). e The colony diameters were larger in LSC cultured on PLA feeder cells. f CFA results are presented as mean ± SE from at least three independent experiments. Statistical analysis was performed using two-tailed Student’s t tests (**p < 0.01; ***p < 0.001). CFA, colony forming assay; D, day; DPT, doubling population time; LSC, limbal stem cells; LSC-3T3, co-cultures of LSC with 3T3 feeder layers; LSC-PLA, co-cultures of LSC with PLA feeder layers; PLA, processed lipoaspirate cells