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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Xenofree generation of limbal stem cells for ocular surface advanced cell therapy

Fig. 7

Expression of stemness and differentiation markers in LSC cultured with PLA and 3T3 feeder layers. a Analysis of the mRNA expression levels for CK3 and CK12 (corneal epithelial differentiation markers), and Bmi1, ABCG2, CK19, CK15 (putative LSC markers), and PAX6. There was minimal differences between LSC cultured on PLA or 3T3 feeder layers. b Immunofluorescence negative for CK12 and CK3. Results are presented as mean ± SE from three independent experiments. Statistical analysis was performed using two-tailed Student’s t tests (*p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant). CK, cytokeratin; CO, corneal epithelial cells; LSC, limbal stem cells; LSC-3T3, co-cultures of LSC with 3T3 feeder layers; LSC-PLA, co-cultures of LSC with PLA feeder layers; PLA, processed lipoaspirate cells. Bar = 25 μm

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