Fig. 4

Type I aggregates present hair peg-like structures. a–i Three-day dermal-epidermal cell aggregates from Fig. 1a were harvested and processed for H&E staining for histological analysis and for IF staining (red or green) for analysis of the expression of proteins as indicated. Nuclei are stained with DAPI (blue); black arrows indicate epithelized structures; white arrows indicate positive staining of the corresponding proteins. Bars = 50 μm