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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Modeling appendicular skeletal cartilage development with modified high-density micromass cultures of adult human bone marrow-derived mesenchymal progenitor cells

Fig. 1

Schematic of chondrogenic organotypic culture model. a Preparation of gelMA overlay, high-density hBM-MPC micromass cultures: A 2-μL droplet of 20 × 106 cells/ml hBM-MPC s was placed on the texturized and collagen-coated surface cultures. After 30 min of cell attachment, a 100-μL aliquot of photocrosslinked gelMA (5%) was placed on top of the culture, which kept the engineered tissue flat during the 14-day experiment. In some experiments, 1/100 of the cells in the micromass culture consisted of hBM-MPCs transduced with a lentiviral COL2A1 promoter-driven GFP reporter, applicable for non-invasive assessment of chondrogenesis. b, c Alcian blue staining of day 14 hBM-MPC micromass cultures (b) with and (c) without gelMA overlay, showing differences in culture morphology and GAG production

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