Fig. 3

G5-cRGD-mediated siRNA delivery in an SSC line. a Intracellular localization of nano-siRNA complexes by confocal laser scanning microscope (CLSM). Images were taken when cells were incubated for 6 h. The nuclei were stained blue by Hoechst 33342, and the green spots refer to internalized nano-siRNA complexes. The scale bar is 10 μm. b Transfection efficiencies of nano-siRNA complexes at various N/P ratios by flow cytometry after 6 h of incubation. Flow cytometry histograms (c) and bar chart (d) indicate the transfection efficiency of different FAM-siRNA formulations at the N/P ratio of 10. e Mean fluorescence intensity (MFI) of G5, G5-cRGD, and Lipo2000 with FAM-siRNA (N/P 10:1) determined by flow cytometry. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s test. Data are presented as mean ± standard deviation (SD, n = 3). *p < 0.05, **p < 0.01