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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: PAMAM-cRGD mediating efficient siRNA delivery to spermatogonial stem cells

Fig. 4

Gene silencing efficiency and cell proliferation assay in C18-4 cell line. The Relative Cdk1 (a) and Cdk2 (b) mRNA expression after treatment with siRNA against Cdk1 (50 nM) and Cdk2 (50 nM) compared with the Mock group in cells at the N/P ratio of 10. Lipo2000 was used as a positive control. c Cell viability was assessed by the CCK-8 assay at 48 h post-treatment. d CDK1 and CDK2 level was detected by Western blot. The expression of PCNA at the protein level indicates the status of proliferation. Positive control: whole testes lysates. e Intensity analysis of CDK1, CDK2, and PCNA were calculated by Image-Pro Plus. g Bar graphs of cell multiplication upon (f) EdU incorporation assay were shown as the ratio of EdU-positive cells. Scale bars: 100 μm. Statistical significance was assessed using one-way ANOVA or Student’s t test. Data are presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01 compared with NC in the same vector group; #p < 0.05, ##p < 0.01 compared with KD-(Cdk1+Cdk2) in different vector group. Mock: untreated group; NC: scramble siRNA group; KD-(Cdk1+Cdk2): Cdk1+Cdk2 siRNA group

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