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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: PAMAM-cRGD mediating efficient siRNA delivery to spermatogonial stem cells

Fig. 5

Purification and characterization of primary SSCs. a Flow cytometry data to illustrate the gating strategy for FACS purification of CDH1+ germ cells. b Immunocytochemical staining of SSC colonies on Sertoli cell feeder layer after 14 days of culture. The fluorescence staining is performed on CDH1 FACS sorted cells following differential plating. Expression of CDH1, Lin28, and PLZF in SSC colonies was showed in green fluorescence. Negative (no primary) control: omission of primary antibody. The nuclei (blue) were stained with Hoechst 33342. Scale bar: 100 μm

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