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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: PAMAM-cRGD mediating efficient siRNA delivery to spermatogonial stem cells

Fig. 6

G5-cRGD-mediated siRNA delivery in primary SSCs. a Localization of FAM-siRNA with G5 or G5-cRGD as carries was examined by CLSM 12 h after the transfection. Labeled siRNA was located in the THY1-positive cell cytoplasm. Red: THY1 staining used to mark SSCs; green: FAM-labeled siRNA; blue: Hoechst 33342 used to stain nuclei. Scale bars: 10 μm. b Fluorescent photographs were prepared 12 h after transfection with different FAM-siRNA formulations. Scale bars: 50 μm. c Percentages of FAM-positive cells among the THY1-positive cells were determined from 20 randomly selected regions by three independent experiments. Suppression of Cdk1 (d) and Cdk2 (e) using the G5-cRGD delivery vector was examined in primary SSCs at the mRNA level. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s test. Data are presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01. Mock: untreated group; NC: scramble siRNA group; KD-(Cdk1+Cdk2): Cdk1+Cdk2 siRNA group

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