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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Age-dependent impairment of adipose-derived stem cells isolated from horses

Fig. 1

Analysis of proliferation of ASCs for the three age donor groups (0–5 years, 5–15 years, > 15 years). a Cell proliferation was assessed, and b PDT was quantified using resazurin-based TOX-8 assay. The red asterisk refers to the comparison of the < 5 and 5–15 groups to > 15, whereas the green asterisk refers to the comparison of the < 5 and < 15 groups to 5–15. c Relative expression of proliferation-associated miRNAs was detected by the qRT-PCR method. d Representative images of immunofluorescence staining for Ki-67 (red) and nuclei (blue). Ki-67 expression in cells was presented as Ki-67-positive cell percentage. A positive colocalization of Ki-67 in the nucleus is indicated by pink signals (merge) due to overlap of Atto 590 (Ki-67) and DAPI staining. Results expressed as mean ± SD. Statistical significance is indicated with asterisks: *p < 0.05, **p < 0.01, ***p < 0.001 using one-way ANOVA (and non-parametric) test

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