Fig. 10
From: Age-dependent impairment of adipose-derived stem cells isolated from horses
Age-associated changes in the expressions of UPR-linked markers. Expressions of CHOP (a), PERK (b), eIF2α (c), BiP (d), ATF6, (e), and IRE1 (f) were determined using qRT-PCR. g To evaluate the mRNA levels of uXBP1 and sXBP1, RT-PCR was performed with XBP1 primers, and the PCR products were run in the 2% agarose gel. Relative quantity of uXBP1 and sXBP1 was determined using Image Lab software after normalization with GAPDH as a reference gene. Results expressed as mean ± SD. Statistical significance is indicated with asterisks: *p < 0.05, **p < 0.01, ***p < 0.001 using one-way ANOVA (and non-parametric) test. h Representative confocal microscopy images of PDIA3 staining