Fig. 2
From: Age-dependent impairment of adipose-derived stem cells isolated from horses
Morphological alternations caused by aging in ASCs. a Representative images of senescence-associated SA-β-gal assay. Black arrows indicate SA-β-gal-positive cells (blue), whereas white arrows show binucleated cells. b Quantification of SA-β-gal-positive cells (blue). c Representative images of Phalloidin Atto 590 staining for F-actin. d SEM images of ASCs isolated from the three age groups (ASC< 5, ASC5–15, ASC15< ). e Mean diameter of the cell nuclei measured on the basis of SEM images. Results expressed as mean ± SD. Statistical significance is indicated with asterisks: *p < 0.05, **p < 0.01, ***p < 0.001 using one-way ANOVA (and non-parametric) test