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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Age-dependent impairment of adipose-derived stem cells isolated from horses

Fig. 3

Age-associated changes in the cell cycle distribution (a, b), protein level of Oct 3/4 (c), mRNA level of CXCR4 (d), accumulation of γH2AX (c), expression of DNMT1 (f), and transcript levels of TET-2 and TET-3 (g, h). Cell cycle distribution was determined using a flow cytometry-based system Muse™ Cell Analyzer. The levels of Oct 3/4, γH2AX, and DNMT1 were estimated with the western blot method. Relative quantity of proteins was estimated using Image Lab software after normalization with β-actin (loading control). Representative blots are shown on the bottom panel. Alternations in the mRNA expressions of CXCR4 (c), TET-2 (g), and TET-3 (h) were determined using qRT-PCR. Results expressed as mean ± SD. Statistical significance is indicated with asterisks:*p < 0.05, **p < 0.01, ***p < 0.001 using one-way ANOVA (and non-parametric) test

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