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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Age-dependent impairment of adipose-derived stem cells isolated from horses

Fig. 7

Analysis of mitochondria dynamics in ASCs using qRT-PCR and western blot techniques. Relative mRNA expression of MFN1 (a), FIS1 (b), PINK1 (c), and PARKIN (d) determined by qRT-PCR. e, f Protein levels of MFN1 and MFF were estimated using western blot. Relative quantity was determined using Image Lab software after normalization with β-actin as a loading control. Representative blots are shown in the bottom panels. Results expressed as mean ± SD. Statistical significance is indicated with asterisks: *p < 0.05, **p < 0.01, ***p < 0.001 using one-way ANOVA (and non-parametric) test

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