Fig. 9
From: Age-dependent impairment of adipose-derived stem cells isolated from horses
Age-associated alternations in the expressions of insulin resistance-related markers. Relative transcript levels of IRS (a), SREBP-1C (b), SIRT1 (c), GLUT-4 (d), and FOXO1 (e) were determined using the qRT-PCR technique. Protein contents of GLUT-4 (f) and INSR (g) were evaluated with the western blot method. β-Actin was used as a loading control. Results expressed as mean ± SD. Statistical significance is indicated with asterisks: *p < 0.05, **p < 0.01, ***p < 0.001 using one-way ANOVA (and non-parametric) test. h Representative images of immunofluorescence staining for GLUT-4 in equine ASCs from the three age-matched groups