Fig. 7

TX co-treatment increases both PDL-1 expression and indoleamine 2,3-dioxygenase secretion. MIAMI cells were treated with IFN-γ with or without 5 μM TX or 10 μM CQ for 4 days to evaluate the effect of the treatments on expression of PD-L1 protein using flow cytometry analysis. Graphs are a representation of six independent experiments (a). MIAMI cells were co-cultured with activated CD4+ T cells in the presence of 5 μM TX or 10 μM CQ for 3 days to determine IDO expression in culture supernatants. ELISA analysis in culture supernatants was used to quantify IDO secretion (b), and IL-6 ELISA analysis in culture supernatants was used to quantify IL-6 secretion (c). “*” ≤ 0.05, “**” ≤ 0.01