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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Bone marrow-derived neural crest precursors improve nerve defect repair partially through secreted trophic factors

Fig. 1

Characterization and tracking of rBM-NCPs. a The rBM-NCPs in adherent monolayer culture on PLL-coated plates showed round or short-spindle shape. b Immunofluorescent staining of rBM-NCPs demonstrated positive expression of neural crest markers CD133 (red), p75 (red), and Nestin (red), and cell nuclei were labeled with DAPI (blue). c Immunofluorescent staining of rBM-NCPs demonstrated positive expression of neural crest markers Vimentin (green in left panel) or CD29 (green in right panel) with proliferation marker Ki67 (red) and DAPI (blue) labeled cell nuclei. d Induced Schwann cells from differentiated rBM-NCPs showed spindle-like shape with a side-by-side alignment. e Induced Schwann cells demonstrated positive expression of Schwann cell markers S100β (red), GFAP (green), and p75(red), and cell nuclei were labeled with DAPI (blue). f The rBM-NCPs in adherent monolayer culture were labeled with Qdot-tracker 565 (green) in vitro (left panel) and detectable in frozen sections of TENG after transplantation for 1 week with local magnification (right panel). Scale bars, 25 μm (a, b, c), 50 μm (d, e), and 100 μm (f)

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