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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Long non-coding RNA FER1L4 promotes osteogenic differentiation of human periodontal ligament stromal cells via miR-874-3p and vascular endothelial growth factor A

Fig. 2

FER1L4 promoted the osteogenic differentiation of PDLSCs. Cells were transfected with a vector expressing FER1L4 (pQLL-FER1L4), siRNA targeting FER1L4 (siFER1L4), or their controls. a Images of ALP staining on day 7 of osteogenic differentiation and Alizarin red S staining on day 14 of osteogenic differentiation in the pQLL-NC and pQLL-FER1L4 groups. Scale bar, 100 μm. Histograms show ALP activity and quantification of Alizarin red S staining by spectrophotometry. b Images of ALP staining and Alizarin red S staining in the siNC and siFER1L4 groups. Scale bar, 100 μm. Histograms show ALP activity and quantification of Alizarin red S staining by spectrophotometry. Results are presented as mean ± SD (*p < 0.05, **p < 0.01)

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