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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Long non-coding RNA FER1L4 promotes osteogenic differentiation of human periodontal ligament stromal cells via miR-874-3p and vascular endothelial growth factor A

Fig. 6

VEGFA was a target of the FER1L4/miR-874-3p regulatory network during osteogenesis. a Schematic diagram of the miR-874-3p putative binding site in WT and MU VEGFA 3′-UTR. b The relative luciferase activities of luciferase reporters containing WT or MU VEGFA 3′-UTR in PDLSCs and 293T cells transfected with miR-874-3p. c Relative mRNA expression of VEGFA in PDLSCs overexpressing miR-874-3p or FER1L4. d Western blot analyses of VEGFA in PDLSCs overexpressing miR-874-3p or FER1L4. Histograms show quantification of the band intensities. Results are presented as mean ± SD (*p < 0.05)

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