Fig. 2

Poly(I:C) transfection upregulates mRNA expression of IFN-β and λ1 in hNSCs. a RT-qPCR analysis of IFN-β, α, and λ1 expression in poly(I:C)-transfected hNSCs (1 μg). The experiments were triplicated, and the error bars represented the standard deviation (SD). b Human NSCs were transfected with 0.2, 1, and 2 μg of poly(I:C) by Lipofectamine 2000 (LF2K), and the expression of IFN-β and IFN-λ1 was analyzed by RT-qPCR. The experiments were performed in triplicate, and the error bars represented the SD. c The supernatants of human NSCs transfected with 1 μg poly(I:C) were harvested at 6, 12, and 24 h post-transfection, and the protein expression of IFN-β was determined using ELISA. The experiments were performed in triplicate, and the error bars represented the SD. Student’s t test was used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001. d Western blot analysis was applied to examine the expression of phospho-IRF3 (p-IRF3) and total IRF3 at different time points in poly(I:C)-transfected hNSCs (1 μg). e Immunofluorescence images of p-IRF3 and nestin in human NSCs transfected with LF2K only (a) and poly(I:C) (b). The cell nuclei were counterstained with DAPI. Scale bar = 20 μm. f Western blot analysis was applied to examine the expression of phospho-IRF7 (p-IRF7) at different time points in poly(I:C)-transfected hNSCs (1 μg). β-actin was used as an internal control