Fig. 6

Transcription of IFN-β expression is increased in ZIKV- and JEV-infected hNSCs. a Human NSCs were infected with Zika virus at an MOI of 1, and RT-qPCR was applied to detect the mRNA levels of Zika virus RNA (vRNA), IFN-β, and IFN-λ1 at indicated time points. b RT-qPCR analysis was performed to examine the mRNA expression levels of JEV virus RNA, IFN-β, and IFN-λ1 in hNSCs that infected by JEV at an MOI of 1. c Human NSCs were transfected with siRNA targeting RIG-I or MDA5 and then infected with Zika virus at an MOI of 1 for 48 h. RT-qPCR was used to analyze the relative amounts of IFN-β mRNA, and western blot assay was performed to confirm the knockdown efficiency. d Human NSCs transfected with siRNA targeting RIG-I or MDA5 were infected with JEV at an MOI of 1 for 48 h. RT-qPCR analysis was performed to analyze the expression of IFN-β mRNA, and western blot assay was used to confirm the knockdown efficiency. The experiments were performed in triplicate, and the error bars represented the SD. Student’s t test was used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001. n.s., non-significant