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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: LncRNA KLF3-AS1 in human mesenchymal stem cell-derived exosomes ameliorates pyroptosis of cardiomyocytes and myocardial infarction through miR-138-5p/Sirt1 axis

Fig. 6

KLF3-AS1 secreted by human mesenchymal stem cells hampers pyroptosis of cardiomyocytes by downregulating miR-138-5p. Note: After cells were transfected with miR-138-5p inhibitor or incubated with KLF3-AS1 exosomes, the expressions of miR-138-5p and KLF3-AS1 were measured by qRT-PCR (a, b). The expressions of NLRP3, ASC, caspase-1/cleaved-caspase-1, and GSDMD/cleaved-GSDMD in hypoxia cardiomyocytes were determined by both qRT-PCR (c) and Western blot (d). Cell apoptosis was determined by TUNEL staining, and DAPI was used to stain cell nucleus (× 200) (e). Inflammatory response was measured by detecting expressions of IL-1β and IL-18 using ELISA (f). Cell viability was determined by CCK-8 on cell viability (g). *P < 0.05, **P < 0.01, vs NC exosome + NC inhibitor group; #P < 0.05, ##P < 0.05, vs NC exosome + inhibitor group; qRT-PCR, quantitative reverse transcription-polymerase chain reaction

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