Fig. 7

KLF3-AS1 secreted by human mesenchymal stem cells hampers pyroptosis of cardiomyocytes by upregulating Sirt1. Note: Hypoxia cardiomyocytes were treated with sh-sirt1 or/and KLF3-AS1 exosome. qRT-PCR (a) and Western blot (b) showed that KLF3-AS1 exosome could upregulate while sh-sirt1 could downregulate expression of Sirt1. The effect of Sirt1 knock down and KLF3-AS1 exosome on mRNA and protein expressions of NLRP3, ASC, caspase-1/cleaved-caspase-1, and GSDMD/cleaved-GSDMD was determined by qRT-PCR (c) and Western blot (d). Cell apoptosis of hypoxia cardiomyocytes was determined by TUNEL staining (× 200) (e). Concentration of IL-1β and IL-18 was determined by ELISA (f). Cell viability was determined by CCK-8 (g). *P < 0.05, **P < 0.01, vs NC exosome + sh-NC group; ^#P < 0.05, ^##P < 0.01, vs NC exosome + sh-Sirt1 group; qRT-PCR, quantitative reverse transcription-polymerase chain reaction