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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Pro-inflammatory cytokines induce cell death, inflammatory responses, and endoplasmic reticulum stress in human iPSC-derived beta cells

Fig. 1

Differentiation of control iPSCs into pancreatic endocrine cells. Control iPSCs (HEL115.6) were differentiated into pancreatic endocrine cells using a 7-step protocol. At the end of stage 7, a mixed cell population comprised of insulin- (Ins), glucagon- (Gcg), somatostatin-positive cells (Sst), and polyhormonal and non-hormonal cells was obtained. Whole aggregates (a) or dispersed aggregates (b) were fixed and expression of insulin, somatostatin and glucagon was assessed (Ins green, Gcg red, Sst purple). Nuclei were counterstained with Pdx1 immunostaining (a) or DAPI (blue, b). Pictures were taken with an epifluorescence microscope with × 20 (scale bar = 50 μm) magnification

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Stem Cell Research & Therapy

ISSN: 1757-6512