Fig. 4

IFNγ + IL-1β and IFNα induce gene expression changes in iPSC-derived pancreatic endocrine cells. Control iPSCs (HEL115.6) were differentiated into pancreatic endocrine cells and exposed to IFNγ + IL-1β or IFNα for 24 and 48 h. a–h The expression of HLA-ABC, Cxcl10, Ins, Gcg, Nkx2-2, MafA, Pdx1, Nkx6-1, and Actin (reference gene) was quantified by RT-qPCR. Expression was corrected for the corresponding actin value and expressed as fold change compared to untreated cells (control) (n = 3–6 independent experiments). i CXCL10 secretion to the culture medium was quantified by ELISA. Data were calculated as pg/mL CXCL10 in the medium and normalized for total protein content (n = 8 independent experiments). *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001 (one-way ANOVA followed by paired Student’s t test)