Fig. 7

Time course analysis of IFNγ + IL-1β- or IFNα-induced STAT1, STAT2, and PDL1 expression in iPSC-derived pancreatic endocrine cells. Control iPSCs (HEL115.6) were differentiated into pancreatic endocrine cells and exposed to IFNγ + IL-1β (a–c) or IFNα (d–g) for 0, 1, 2, 4, 8, 24, and 48 h. Total proteins were extracted, and expression of pSTAT1 (a, d), total STAT1 (b, e), PDL1 (c, g), and pSTAT2 (f) were assessed by Western blotting. GAPDH was used as a control for protein loading. Protein signals were quantified and corrected for the corresponding GAPDH value and expressed as fold change compared to untreated cells (CTL) (n = 3–4 independent experiments). *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001 (unpaired Student’s t test; significantly different from the control condition at the same time point)